Background: Rheumatoid arthritis (RA) is a chronic autoimmune disorder that primarily affects the joints, causing inflammation, pain, swelling, and potentially leading to joint deformities and disability over time. The present study was conducted to assess disease activity score with respect to vitamin D in rheumatoid arthritis. Materials & Methods: 78 patients of rheumatoid arthritis of both genders were selected and measurement of vitamin D was done with ELISA in an autoanalyzer. Disease activity score 28 (DAS28) and Visual analog scale scoring system tools were used. Results: Out of 78 patients, males were 48 and females were 30. The mean vitamin D level and DAS- 28 score in patients with low disease activity was 10.2 ng/ml and 2.5, in moderate disease activity was 11.0 ng/ml and 4.1, in high disease activity group was 10.7 ng/ml and 5. The difference was significant (P< 0.05). Conclusion: A strong correlation between the DAS-28 score and vitamin D level has been discovered.