Background: High grade glioma resection under fluorescein guidance have shown improved visualization of tumor margin and has improved the extent of resection. Methods: This is a single centre retrospective analysis of 69 patients of high grade glioma operated under fluorescein guidance with operating microscope using yellow filter. The data was analyzed from December 2021 to December 2022 and was evaluated for surgical outcome interms of residual tumor and post operative residual volume using T1 weighted contrast MRI (extent of excision), post operative complications with 6 month follow up. Intravenousfluorescein sodium (2mg/kg) was administered at the time of incision. Results: None of the patient had any adverse reaction to fluorescein. The mean preoperative tumor volume was 29.4cm3. Gross Total Resection (GTR) was achieved in 55 patients (79.76%) and STR in 14 patients (20.24%) with mean postoperative residual tumor volume 1.63cm3 (range 0.11 – 13.4cm3). 11 patients (15.9%) had Grade 3 and 58 (84.05%) patients had Grade 4 gliomaas per WHO classification, 5th Edition. In post operative period 1 patient (1.14%) had multiple episodes of seizures, 3 patients (4.34%) had lower respiratory tract infection (LRTI), 5 patients(7.24%) developed wound infection and 3 patients died in post operative period during hospital stay, but it was not procedure related mortality. 59 patients (85.5%) had progressionfree survival at 6 month duration with average KPS of 82.5, 8 (11.59%) patients had recurrenceat 6 month follow up, 2 (2.89%) patient died at 6 month follow up. Conclusion: FL guided excision of High grade glioma improves the extent of resection and helps in improving survival.