Background: The current investigation was undertaken to evaluate changes in serum bilirubin concentrations before and after undergoing Laparoscopic cholecystectomy (LC). Materials & methods: A cohort of 100 patients who were scheduled to undergo laparoscopic cholecystectomy (LC) were included in the study. Comprehensive demographic information of all the patients was acquired. Blood samples were collected and the levels of serum bilirubin were evaluated prior to the surgical procedure at the initial stage. The serum bilirubin profile was reassessed at 24 and 72 hours postoperatively. A comparison was conducted about the changes observed in the levels of serum bilirubin. The data were recorded and analyzed using the SPSS software. Results: Statistically significant differences were observed when comparing the mean total serum bilirubin levels during the preoperative period, postoperative 24 hours, and postoperative 72 hours, which were measured at 0.76 mg/dL, 1.21 mg/dL, and 0.79 mg/dL, respectively. Statistically significant differences were seen in the mean serum direct bilirubin levels during the preoperative period, postoperative 24 hours, and postoperative 72 hours. Specifically, the mean serum direct bilirubin levels were measured at 0.35 mg/dL, 0.69 mg/dL, and 0.39 mg/dL, respectively. Conclusion: There is a notable variation in serum bilirubin levels observed in individuals who have Laparoscopic cholecystectomy.