Background: The present study was conducted for assessingantibiotic sensitivity pattern among diabetic females with asymptomatic bacteriuria. Materials & methods: Assessment of 100 diabetic female subjects was done. Collection of Mid-stream urine samples was carried out and sent within one hour for processing. Microbiological profile and its Antibiotic sensitivity pattern were analyzed. Results: The overall prevalence of asymptomatic bacteriuria among diabetic females was 20 percent. Among these 40 subjects, Escherichia coli, Klebsiella pneumonia, Coagulase negative staphylococciE. coli was mainly resistant to Cephalotin, Ceftriaxone, nalidixic acid and cotrimoxazole. Klebsiella pneumonia was mainly resistant to Ciprofloxacin, Cephalotin, Ceftriaxoneand ampicillin. Coagulase negative staphylococci was mainly resistant to Gentamicin, Ciprofloxacin and Cephalotin while staphylococcus aureus was mainly resistant to Ciprofloxacin and Erythromycin. Conclusion: Appropriate empirical therapy for AUB can be instituted timely on the basis of antipoetic sensitivity and resistance pattern.