Introduction: Dengue is an endemic arboviral disease. Due to the increasing prevalence of dengue infection, early diagnostic confirmation of dengue infection in patients is beneficial for timely clinical intervention, etiological research, and disease management. Evaluation of the Dengue Day 1 test, a commercially available serological test kit. This is for the differential detection of IgM and IgG antibodies in a single acute serum sample intended for detection of Dengue NS1 antigen. Material and Methods: The present study was conducted at Department of Microbiology, Govt. Medical College Doda. This study included a total of 700 participants with acute febrile illness. Serum samples were assayed for dengue NS1 Ag, IgM and IgG antibodies using a dengue day 1 assay, a commercially available rapid solid-phase immunochromatographic assay. Results: 177 of 700 patients had an acute dengue infection. Of 177, 86 (NS1) tested positive for IgM. Infection rates were higher in the 21-30 age group and men were more affected than women. More dengue cases occurred during and after the rainy season. Conclusion: It is recommended that serological tests (NS1Ag, IgM and IgG Ab) are performed early in all cases of suspected dengue so that we can quickly diagnose the disease and initiate the necessary treatment. The current study showed that dengue serological tests play an important role in the early detection of dengue fever.