Background and aim: Fever is one of the most frequent conditions that prompt patients under the age of five to attend the OPD. In comparison to other illnesses that receive greater attention, urinary tract infections (UTIs) as the cause of fever receive very less attention. Antibiotics are frequently administered to children empirically without a proper UTI evaluation. Identification of UTI in children with fever is crucial in order to reduce lifetime morbidity and give timely treatment. The purpose of the current study was to evaluate the prevalence of urinary tract infections (UTIs) in children under the age of five and the reliability of urine culture and analysis in the diagnosis of UTIs. Methods: 120 participants between the ages of 2 months and 5 years who were hospitalized for fever were evaluated in the current prospective clinical investigation. Demographics and predisposing variables were recorded for each individual. Participants older than 2 years old had clean midstream pee collected, whereas younger participants had urine samples taken in bags. Urine culture and analysis were performed on all participants, and an ultrasonogram was performed on those whose cultures were positive. Results: In the present study, Klebsiella, Proteus, pseudomonas, and E. coli were isolated and observed for culture growth. Sensitivity to Cefoperazone, Amikacin, Cefotaxin, Nitrofurantoin, and Gentamycin was observed. In the subjects of the current study who tested positive for culture, the ultrasound revealed hepatomegaly in 2 males, bilateral hydronephrosis with obstruction of the PUJ in 1 female, bilateral hydronephrosis with thickening of the bladder wall in 1 female but not in any male subjects, and cystitis in 2 female and 1 male subjects. Conclusion: In order to reduce long-term complications, sequelae, and morbidity, the current study's findings suggest that subjects with significant pyuria—defined as >5pus cells/HPF in the urine sample—should be assessed further and promptly begin receiving antibiotic therapy for UTIs.