Background: To examine the value of several biomarkers in serum including lactate dehydrogenase (LDH), glutamate oxaloacetate transaminase (SGOT), creatinine phosphokinase (CPK), alkaline phosphatase and phosphorus sample for the early diagnosis of acute mesenteric ischemia. Materials & methods: 50 patients collected were grouped as group A and group B, according to the presence or absence of ischemia as per finding of intra operative/angiography. Total 25 patients were the part of group A with intra operative finding of presence of mesenteric ischemia and patients strongly suspected to have acute mesenteric ischemia on imaging and confirmed by angiography if not operated. While equal number of patients (n=25) were recruited in group B with mesenteric ischemia and without intra operative/angiographic finding. In both the groups the levels of above enlisted enzymes were estimated. Results: The distribution of LDH levels among the cases studied did not differ significantly between two study groups (P-value>0.05).The distribution of SGOT levels among the cases studied did not differ significantly between two study groups (P-value>0.05).The distribution of CPK levels among the cases studied differs significantly between two study groups (P-value<0.05). Significantly higher proportion of cases in Group A had raised CPK levels compared to group of cases from Group B (P-value<0.05).The distribution of ALP levels among the cases studied differs significantly between two study groups (P-value<0.01). Significantly higher proportion of cases in Group A had raised ALP levels compared to group of cases from Group B (P-value<0.01). The distribution of sr. phosphorous levels among the cases studied differs significantly between two study groups (P-value<0.05). Significantly higher proportion of cases in Group A had raised sr. phosphorous levels compared to group of cases from Group B (P-value<0.05). Conclusion:The observation of all the serum markers done in the cases of acute abdomen have shown to be of help in early predicting of mesenteric ischemia. Serum marker levels also aids in determining the underlying severity of intestinal gangrene/ necrosis based on the levels at which it’s raised.