Background: The paediatricians in the private sector in India can play an important role in providing vaccine service delivery and immunization coverage. Standards and systems for service quality of private providers should be established by countries.Standards should include practices in all facilities delivering vaccines, including proper storage and handling, appropriate use of injections, proper recording and adherence to safety measures, and waste management and disposal. [1]There is also a need to have co-partnership and communication with private providers to improve the performance of health system in long term. Methodology: A cross sectional study was conducted in urban area of Bhopal city. Knowledge and adherence to standard guidelines related to vaccination practices were assessed. Total 110 paediatricians were found eligible for the present study.After excluding 10 paediatricians who refused to participate, finally, 100 paediatricians were involved(responded) giving response rate of 90.9%. Pre-designed;pretested questionnaire was used for data collection. Results: In this study, the study population were paediatricians providing vaccination in private clinics and private hospital. Most of the private providers 50% were in between 41 to 60 age group, 81% paediatricians were trained and 82% immunization clinics were registered to Government sector. Out of total respondents (76/100) 76% answered correctly on all knowledge item questions. Most respondents 76% had complete knowledge score on cold chain vaccine. Mean (SD) for knowledge score was 96.3(±7.61) ranging from 70 to100. Conclusion: Knowledge of majority of paediatricians were good, for success of NIP (National Immunization Program),it is necessary to increase the private sector involvement in the area of immunization delivery.