Aim: Present study aimed to study the Microbiological profile of dermatophytic infections and to identify the causative fungal species in various clinical presentations. Materials and Method: 752 clinically suspected cases of dermatophytosis were randomly selected for the study. Details of skin lesions were noted. Depending on the presenting condition skin scales, crusts, nail clippings, pluckable hair were collected. After direct microscopic examination, irrespective of demonstration of fungal elements, the specimen was inoculated into a test tube containing Sabouraud’s dextrose agar with 0.05% chloramphenicol and 0.5% cycloheximide. This was incubated at 28° C for up to 4 weeks. If no growth was found after 4 weeks, it was taken as negative for growth of fungi. Fungal isolates were identified based on colony morphology, pigmentation, growth rate, microscopy in Lactophenol Cotton Blue mount(LCB). The statistical analysis was done on Microsoft Word and Excel. Results: Tinea cruris(51%) was the most common type with cases followed by tinea corporis(40%) cases and tinea faciei(4%) cases and tinea mannum(3%) cases. Out of 752 clinically suspected cases of dermatophytosis, fungi were demonstrated in 465 (61.83%) cases by direct microscopy and 120 (13.5%) were culture positive. There is a significant association between KOH and culture as all 120 culture positive samples had fungi demonstrated in direct microscopy. Among these 102 cases, the most common isolate was T. mentagrophytes with 32 cases (31.37%), followed by T. tonsurans with 28 cases (27.45%) and T.verrucosum with 26 cases (21.66%). Single specie of each namely T.voilecium and Microsporum was isolated. Malasezia cases were 4 (3.33%). Mixed infection with Tricophyton species was observed in single case. Diabetes mellitus was the most common systemic disorder with dermatophytosis with 20(19.60%) cases followed by HIV infection with 5(4.9%) cases. Conclusion: The present study also reveals the changing trends in the prevalence of various dermatophyte species in this part of Bihar. There is raised incidences of dermatophytosis in Females which can be corelated with their increased participation in outdoor activities, work and raised medical concern for diagnosis and treatment. Finally, the clinicians should always consider the risk of multisite involvement by fungi causing dermatophytosis in patients associated with systemic diseases. General awareness of personal hygiene avoiding delay in seeking treatment should be propagated by medical professionals.