Background: Vitamin D is a lipid-soluble vitamin that is known to play an important role in bone metabolism through the regulation of calcium and phosphate homeostasis. Thus, insufficiency or deficiency of this vitamin can have long-term effects, particularly for children. The present study was conducted to compare vitamin D status of children younger and older than 2 years. Material and Methods: The present study was conducted among 200 healthy children younger and older than 2 years to compare Vitamin D Status in them. Children were divided into two groups i.e. group A was children younger than 2 years and group B was children older than 2 years. Blood levels of vitamin D were then measured. The data were analyzed using the statistical package for social sciences (SPSS), version 16 (SPSS Inc. Chicago, IL, USA) for Windows. A P value of less than 0.05 considered statistically significant. Results: In the present study children were divided into two groups i.e. group A was children younger than 2 years and group B was children older than 2 years. 60% children younger than 2 years were males and 40% were females whereas 55% children older than 2 years were males and 45% were females. Mean age of children younger than 2years was 1.23 years and children older than 2 years was 3.45years. Mean levels of Vitamin D was more in children younger than 2 years(28.72ng/ml) than children older than 2 years (16.13ng/ml). In children younger than 2 years, 46 children had normal vitamin D levels, 47 children had insufficient levels and 7 children had deficient vitamin D levels. In children older than 2 years, 15 children had normal vitamin D levels, 43 children had insufficient levels and 42 children had deficient vitamin D levels. In 33.91% males and 25.88% females, vitamin D levels were normal. In 42.60% males and 48.23% females vitamin D levels were insufficient and in 23.47% males and 25.88% females vitamin D levels were deficient. Conclusion: The study concluded that children older than 2 years had more children who had deficient vitamin D levels than children younger than 2 years. Females had less levels of vitamin D than males.