Background: The present study entitled ‘To evaluate the possible association between vitamin D deficiency and pelvic floor disorders’ was conducted in the Department of obstetrics and gynecology over a period of one year. In the present study, 200 women were selected randomly attending OPD clinic or who were admitted in the ward. Women having pelvic floor disorder (PFD) formed Cases and those without PFD were taken as Controls. Aim: To evaluate possible association between Vitamin D levels and Pelvic floor disorders. Material and methods: 200 females above 40 years of age were selected randomly attending OPD clinic or who were admitted in the ward. Women above 40 years of age with or without PFD are included in the study while Women with pre-existing thyroid disorders, parathyroid or calcium metabolism disorders Women who took medications that interfere with calcium or vitamin D metabolism (diuretics or calcium channel blockers),Women suffering from bone renal adrenal or gastrointestinal disorders. Women with history of ATT intake or antiepileptic drugs or on hormone therap are not included in the study. Results: A total of 200 women above the age of 40 years attending OPD or admitted in the ward of Obstetrics and Gynecology Department. were selected for this study. One hundred women with pelvic floor disorder (PFD) were taken as Cases, while the other one hundred without PFD were taken as Controls. Number of women with normal BMI (18.5-24.9 kg/m2) in Cases was 64%, while in Controls it was 74%, while number of underweight women (<18.5 kg/m2) in Cases was 15% compared to only 1% in Controls. Conclusion: Our research strongly suggests a link between Pelvic Floor Disorders (PFD) and Vitamin D deficiency. Early detection of vitamin D deficiency is crucial to initiate timely and adequate treatment, thereby preventing various health issues associated with its deficiency, including PFD.