Aim: The level of the lipid peroxidation biomarker known as MDA in synovial fluid was shown to have a correlation with the severity of primary knee osteoarthritis. Materials and methods: 40 Patients aged 46 and older with acute osteoarthritis symptoms (knee effusion) were included in this study. Knee osteoarthritis was graded using Kellgren-Lawrence (K-L) Radiographic rating system. Grade 1: Dubious, Grade 2: Mild, Grade 3: Moderate and Grade 4: Severe. The Kellgren-Lawrence grading system was used in order to analyse the radiographs of the knee joint. The grading of the knee was connected with oxidative stress measures (the levels of MDA in the synovial fluid), and this was done so that probable correlations between the oxidative stress-induced damage and the course of the illness could be found. Results: The MDA levels of subjects who were in grades 3 and 4 were found to be considerably (p< 0.001) higher than the MDA levels of individuals who were in grades 1 and 2. (Mean MDA 4.44 and 4.69). Tukey's Honestly significant difference procedure was used to conduct pair wise comparisons of the means, which revealed that grade 3 (Mean MDA 5.87) and grade 4 (Mean MDA 6.11) had significant comparisons: subjects in grades 3 and 4 reported that their MDA levels were significantly (p<0.001) higher than subjects in grades 1 and 2 (Mean MDA 4.69). It was determined that there was not a significant difference between grades 1 and 2 (p=0.33). Conclusion: There was a positive relationship between the Kellgren-Lawrence grade and synovial MDA. Antioxidant supplementation in individuals with early osteoarthritis may help delay the progression of the disease by increasing the antioxidant status of the knee. This rise in antioxidant status helps to neutralise the formation of free radicals and prevent the breakdown of cartilage.