Background: Transfusion-related morbidity and mortality are more likely when infectious blood and blood components are used. The present study was conducted to assess seroprevalence of transfusion transmitted infections amongst blood donors. Materials & Methods: 265 donors of both genders were included. The screening tests were performed by following the current national guidelines and the manufacturer’s instructions. The TTI screening section used the electrochemiluminescence immunoassay to screen for HBsAg, anti-HCV, HIV, and anti-TP. The screening for malarial parasite was performed by enzyme-linked immunosorbent assay. Results: Out of 265 patients, males were 155 and females were 110. Type of donation was voluntarily in 92 and replacement in 173. The difference was significant (P< 0.05). Transfusion transmitted infections prevalence in voluntarily donor for HBV, HCV, HIV, Malaria and syphilis was 1.5%, 1.2%, 0.8%, 0.03% and 0.4% and in replacement donor was 2.9%, 1.8%, 0.4%, 0.2% and 0.1% respectively. The difference was significant (P< 0.05). Conclusion: There was high prevalence of transfusion transmitted infections among replacement donor. The seroprevalence of HBV, HCV and HIV was relatively high. Therefore, through the use of a highly sensitive screening assay, blood donation should be screened.