Background: Otomycosis is a chronic or subacute, recurrent, non-infectious, persistent superficial fungal infection of the ear, usually localized to the outer ear. The management of otomycosis depends on knowledge of the microbiology of potential pathogens and an understanding of the clinical presentation. The term otomycosis is used to describe a fungal infection of the ear, i.e. the outer ear canal and the eardrum. This condition presents as a primary infection or develops with an external bacterial infection following antibiotic treatment. This study was designed to determine the clinical symptoms and mycobacterial flora of cases of otomycosis. Materials and Methods: This cross-sectional study included patients attending the Otolaryngology Outpatient Department affiliated with the Department of Microbiology, N.C. Medical College and Hospital, Israna, Panipat, Haryana, India, over a two-year period, from July 2017 to June 2019. Total 100 patients diagnosed with otomycosis were included. The fungal culture and other studies were done in the microbiology laboratory by using standard methods. Results: Patients complained mainly of itching in 100%, followed by pain in 71 cases, purulent ear discharge in 52 cases and hearing loss in 43 cases. A total of 87 cases were caused by Aspergillus species and 7 were caused by Penicilium notatum, the remaining 6 were caused by Candida albicans. Conclusion: Aspergillus species appears to be the most common causative agent of otomycosis, followed by Penicillium notatum and Candida albicans. The most common manifestations of otomycosis are itching, ear pain, and ear discharge.