Background: Ear, nose and throat (ENT) infections are common and have a high incidence. These areas are too close to the brain and eyes and can lead to complications that lead to illness or even death. In addition, the ear, nose, and throat are interrelated and interdependent both anatomically and physiologically. Because they are exposed to the outside environment, their mucous membranes are more easily accessible to pathogens. Thus, pathogenic and non-pathogenic organisms colonize these areas. Nonpathogenic agents are common in the upper respiratory tract and are responsible for respiratory health. Materials and methods: Samples from patients presenting for ENT OPD at N.C. Hospital and Medical College, Israna, Panipat, Haryana, India were isolated for further microbiological evaluation. 100 samples from male and female patients, including 55 ear specimens, 30 and 15 throat swabs and nasal secretions, were examined between April and June 2022 and the samples were analyzed by culturing, direct microscopy and identification of bacteria. Result: Research results have identified 7 bacterial genera, namely Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pneumoniae. Conclusion: Staphylococcus aureus was the most common isolate from ear and nasal swabs, while the majority of throat swabs were negative and, in a small number of Streptococcus pneumoniae was isolated, indirectly showing viral infection rates in cases of throat infections. This demonstrates the importance of culture and sensitivity in these cases before starting antibiotics.