Background: Phototherapy is universally acknowledged as the foremost choice for addressing neonatal jaundice, thanks to its exceptional effectiveness and safety in lowering elevated serum free bilirubin levels and mitigating potential neurotoxic impacts. Hence, this study was conducted to compare two methods of phototherapy for treatment of hyperbilirubinemia in very low birth weight newborns. Materials & Methods: A total of 60 premature neonates were enrolled in the present study. Only those subjects were enrolled which had jaundiced and were admitted to the neonatal ward. All the neonates were healthy and were delivered before thirty fifth week of gestation. All the subjects were randomly divided into two study groups as follows: Group A (Control group): Subjects in which phototherapy initiated during the first six hours after birth and continued up to 96 hours regardless of their TSB (prophylactic group), and Group B (Study group): Subjects in which phototherapy started when the TSB level reached higher than 8 mg/dl and discontinued with the TSB level lower than 5 mg/dl. Assessment of total serum bilirubin levels was done at the beginning, and then every twenty hours. All the results were recorded in Microsoft excel sheet. Results: Mean total bilirubin levels among subjects of group A at 1stday, 2ndday, 3rdday, 4thday, 5thday, 6thday and 7thday was 4.99 mg/dL, 6.96 mg/d L, 7.12 mg/d L, 6.53 mg/d L, 6.66 mg/dL, 6.96 mg/dL and 7.02 mg/dL respectively. Mean total bilirubin levels among subjects of group B at 1stday, 2ndday, 3rdday, 4thday, 5thday, 6thday and 7thday was 4.72 mg/dL, 7.13 mg/d L, 7.92 mg/dL, 8.12 mg/d L, 8.29 mg/dL, 7.28 mg/d L and 7.23 mg/dL respectively. Mean bilirubin levels among subjects of group B were significantly higher in comparison to subjects of group A at 4th day and 5th day. Conclusion: The preventive phototherapy intervention for infants weighing between 1000–1500 g brings about a noteworthy reduction in bilirubin levels on the fourth and fifth days postpartum.