Background: Scrub typhus is a tropical disease caused by the rickettsial bacterium Orientia tsutsugamushi.It commonly affects the kidneys, and the degree of renal involvement can vary among patients; characterized by mild conditions like hematuria or proteinuria. The objective of the present study was to assess the risk factors, clinical characteristics, and laboratory parameters of patients with scrub typhus-induced acute kidney injury (AKI). Methods: This was a retrospective study conducted at a tertiary care hospital in central India, for 2 years, from February 2020 to February 2022 involving patients with scrub typhus infection. The medical records of all patients who were admitted and treated for scrub typhus infection were analyzed. The patients were divided into two groups: AKI and non-AKI. Results: A total of 200 patients were included in this study. There was a significant difference between the mean peak creatinine levels (p<0.001), the higher being in the AKI group than non-AKI group (2.8 mg/dL vs. 0.5 mg/dL). The mean duration of hospital stay was higher in AKI group than non-AKI group (9.2 days vs. 6.3 days), with a significant difference of p=0.011. The number of patients presenting with chronic kidney disease (CKD) was higher in AKI group (12.0%) and non-AKI (6.0%), p=0.007. The duration of hospital stays, age (>65 years), presence of CKD and lower albumin level (<3.5 g/dL) remained independent risk factors for AKI. Conclusion: The presence of underlying CKD, elder age (>65 years), lower serum albumin (3.5 g/dL) level and duration of hospital stay were important risk factors to determine occurrence of AKI.