Introduction: Early diagnosis can improve the outcome of patients with acute myocardial infarction (AMI). However, there are no satisfactory cardiac biomarkers for the diagnosis of AMI within 6 hours of onset of symptoms. Among novel biochemical markers of AMI, heart-type fatty acid binding protein (H-FABP) is of particular interest. Aims and objectives: The study aimed to investigate whether H-FABP measurement provides additional diagnostic value to that of conventional cardiac markers-CPK MB and Troponin I in AMI patients. Material and methods: The study included 300 subjects of AMI (150 STEMI and 150 NSTEMI), attending emergency department within 6 hours after the onset of chest pain .Peripheral venous samples were obtained on admission, post thrombolysis at 60 minutes and after 6 and 12 hours to measure a panel of conventional biomarkers HFABP,CPKMB, and Troponin I. In all the cases, serum H-FABP concentration was measured immunochemically. Results: The sensitivity and negative predictive values of H-FABP on admission ,in patients with AMI on admission were significantly greater than CK-MB and Troponin I but specificity and positive predictive values of Troponin I remained significantly higher (100%) than CK-MB and HFABP in patients with AMI. Conclusion: In conclusion, the diagnostic value of H-FABP is greater than CK-MB and troponin I for the early diagnosis of AMI within first 6 h of chest pain. H-FABP can be used as an additional earliest diagnostic tool for the diagnosis of AMI