Objective: This study is aimed to assess the salivary copper levels in patients with oral leukoplakia and explore its potential diagnostic value for premalignant oral lesions. Materials and Methods: A total of 50 participants were enrolled, including 25 healthy individuals (control group) and 25 individuals diagnosed with oral leukoplakia (case group). Salivary samples were collected, and copper levels were measured. The participants were categorized based on age and gender. Statistical analysis was performed using Student's t-test, and a p-value of less than 0.05 was considered significant. Results: The mean age of the control group was 42.5 years, while the case group had a mean age of 47.3 years. Significant differences were observed in salivary copper levels between the control group (mean: 9.78 ppb) and the case group (mean: 72.54 ppb) (p < 0.001). The case group showed substantially higher salivary copper levels compared to the control group. Gender distribution was balanced in both groups. Conclusion: This study provides evidence of significantly elevated salivary copper levels in patients with oral leukoplakia compared to healthy individuals. Salivary copper assessment holds promise as a non-invasive diagnostic tool for detecting premalignant oral lesions. Further research with larger cohorts and longitudinal designs is warranted to validate these findings and explore the clinical implications of salivary copper assessment in the management of oral leukoplakia.