Background and Aims: This investigation was carried out on individuals suffering from diabetic foot ulcers (DFUs) with the purpose of assessing the clinical characteristics, spectrum of gram negative microbial populations, antibiotic susceptibility, and establishing a pragmatic approach for administering antimicrobial therapy. Materials and Methods:Clinical data, along with specimens of tissue and pus, were procured from a total cohort of 50 patients who had received a diagnosis of diabetic foot ulcers. These samples were handled in strict adherence to the protocols outlined by the Clinical and Laboratory Standards Institute. Both the clinical and microbiological data sets underwent meticulous analysis. Results: The present study, encompassing 50 patients afflicted by diabetic foot ulcers, unveiled a predominance of male subjects. The majority of patients fell within the age range of 50 to 60 years. In the collected samples, Gram-negative microorganisms constituted 70.52% of the total. Notably, Gram negative organisms showcased the most notable receptivity towards Colistin, Imipenem, and Meropenem.. Conclusion: Within the realm of DFUs, Gram-negative bacteria exhibited varying degrees of resilience against frequently employed antibiotics, concurrently demonstrating receptivity to aminoglycosides. These findings suggest that Colistin, Imipenem, and Meropenem.hold promise as potential choices for empirical antibiotic intervention in cases of DFU infections.