Background: Early drug resistance can be identified with the CBNAAT (Cartridge-based nucleic acid amplification Test) in a short amount of time after sample submission. Patients with tuberculosis, both those who have recently been diagnosed as well as those who have relapsed, have been experiencing an exponential spread of drug-resistant TB. The main causes of the development of drug-resistant TB, beyond from the initial drug resistance seen in new cases of pulmonary tuberculosis, include patient non-compliance and unneeded and puzzling regimens prescribed by clinicians in failed instances. Objective: 1) To determine the usage of CBNAATas a tool for definitive diagnosis of Pulmonary Tuberculosis. 2) To access the extent of Rifampicin resistance amongst these freshly diagnosed cases of Pulmonary Tuberculosis. Material and Methods: A total of 275 Sputum samples were obtained from the individuals in western Rajasthan who were clinico-radiologically significant. These were handled at diagnostic facilities equipped to conduct CBNAAT tests. All samples were processed using CBNAAT while adhering to the advised aseptic techniques and PPE (Personal Protective Equipment) criteria. Conclusion: The CBNAAT is one of the most efficient diagnostic methods available in India right now. It could be used to concurrently diagnose medication resistance and pulmonary TB. Compared to the standard sputum smear microscopy, it is more useful. Additionally, it may take the place of traditional culture-based drug sensitivity testing techniques.