Background: The most common cause of HIV disease throughout the world is HIV-I and HIV-2 which was first identified in 1986 in West African patients in which HIV-I subtype C is most prevalent. First case in India was identified in 1986 at Chennai. Objective: To study the correlation of different dermatological conditions with HIV infection and to Significance of ELISA for detection of AIDS (HIV infection) in patient of skin disease. Methods: The study was carried out on the patients of skin diseases attending Skin & V.D. outdoor clinic of L.L.R. & Associated Hospitals, Kanpur. The serum separated from the sample was then tested for antibodies to HIV at zonal blood testing centre, Blood Bank & Department of Pathology, Medical College, Kanpur. Results: Maximum percentage of cases belonged to 21-50 years of age group (77.7%). 5% of cases were recipients of blood transfusion while majority of cases 95% cases had no history of blood transfusion. 11.04% cases accepted for sexual promiscuity while 88.96% denied. This result was obtained despite of repeated enquiries. The clinical diagnosis of cases were candidiasis (28.13%), dermatophytosis (14.17%), genital scabies (12.08%), herpes zoster (11.25%), genital warts (7.08%), molluscumcontagiosum (7.91%) psoriasis (6.25%), syphilis (5.62%), seborrhoeic dermatitis (3.33%) , lichen planus (2.91%) and pyoderma (01.25%). Three cases of widespread candidiasis infection were found to be HIV seropositive. They were poorly responding to treatment. Conclusions: Prevalence of HIV seropositivity is about 1.25% of various skin diseases. However, a larger study with greater number of cases and longer duration is required to find out more concrete results.