Background:The bile under normal conditions is sterile. Bactibilia is a common finding in individuals at high risk or with complicated cholecystolithiasis, including obstruction of the biliary tract, choledocolithiasis. The present study was conducted to assess bacteriology of the gallbladder bile in normal subjects. Material & Methods:The present study was conducted among 40 patients. Patients were divided into three groups. The gallbladder was excised; a portion of the fundus (2 cm3) was removed for hacteriologic analysis. The gallbladder bile samples were analyzed and identification of the bacteria was made. Results:In Group I patients with normal gallbladder showed no bacterial growth either aerobically or anaerobically. In Group II, 80% cultures of gallbladder bile remained negative and 20% cultures were positive. The bacterial flora consisted mainly of enteric bacteria like Escherichia coli, Streptococcus fecalis, and Klebsiella. Mixed flora was seen in only one case. In Group II, gallbladder wall, 30% had positive cultures. Flora was similar as in the bile. In Group III, gallbladder bile, 60% had positive cultures. The same enteric bacteria were present, Escherichia coli, Streptococcus fecalis, and Klebsiella. One patient had mixed flora. No anaerobic bacteria were the Gallbladder found. In Group III, gallbladder wall, 40% had positive cultures. Conclusion:The present study concluded that patients with normal gallbladder showed no bacterial growth either aerobically or anaerobically. Patients with chronic cholecystitis had 20% cultures were positive; Patients with acute cholecystitis, 60% had positive cultures. The present study was conducted to assess bacteriology of the gallbladder bile in normal subjects