Background: The present study compared Latanoprostene Bunod, and 0.5% timolol solutions in lowering intraocular pressure in open-angle glaucoma. Materials & Methods: 50 patients of open-angle glaucoma of both genders were divided into 2 groups of 25each. In group I, patients were prescribed latanoprostand in group II, patients were prescribed timolol maleate. IOP was measured using a topical proparacaine 0.5% as the local anesthetic. Blood pressure and heart rate were measured immediately before IOP measurements. Results: Group I had 15 males and 10 females, group II had 12 males and 13 females. The mean IOP was 25.8 mm Hg in group I, and 24.6 mm Hg in group II. Heart rate was 78.4 bpm in group I, and in group II was in 77.2 bpm. The difference was non- significant (P> 0.05). Adverse events were dry eyes seen in 3, and 2, eye pain in 1 and 3, conjunctival hyperemia in 1, and 0, foreign body sensation in eyes in 1, and 0, and eye irritation in 2, and 1 in group I, and in group II respectively. The difference was non- significant (P> 0.05). Conclusion: In patients with open-angle glaucoma, latanoprostenebunod was superior to timolol in lowering intraocular pressure.