Background:Laparoscopic surgery is widely used for its minimally invasive benefits, but its impact on hepatic function due to CO₂ pneumoperitoneum remains a concern. This study evaluates postoperative hepatic biochemical changes and their clinical significance.Methods:A prospective observational study was conducted on patients undergoing laparoscopic surgery. Liver function tests, including ALT, AST, ALP, total and direct bilirubin, serum albumin, PT, and INR, were measured preoperatively and at 24 and 48 hours postoperatively. Statistical analysis assessed the significance of changes in these parameters.Results:A significant increase in ALT (22.88±8.75 to 35.37±10.42 U/L, p < 0.001) and AST (23.96±8.90 to 39.23±10.38 U/L, p < 0.001) was observed at 24 hours, followed by a decline at 48 hours. Total bilirubin (0.76±0.262 to 1.05±0.286 mg/dL, p < 0.001) and direct bilirubin (0.252±0.0962 to 0.37±0.111 mg/dL, p < 0.001) also increased significantly. ALP showed a mild rise (p = 0.043). PT and INR increased significantly (p < 0.001), indicating transient hepatic synthetic dysfunction. All parameters approached baseline by 48 hours.Conclusion:Laparoscopic surgery causes temporary hepatic biochemical alterations, likely due to pneumoperitoneum-induced perfusion changes. However, these effects are self-limiting and clinically insignificant in healthy individuals. Further research is required for patients with pre-existing liver disease.