Introduction- Staphylococcus aureus is one of the common organism capable of causing a wide range of human diseases. It is a significant human pathogen that causes soft tissue infection, wound infection, and produces the pus. Aim -To study the prevalence and antibiotic sensitivity pattern of Staphylococcus aureus isolated in a Tertiary Care Hospital Objectives- i. To isolate Staphylococcus aureus and study the prevalence rate. ii. To study Antibiotic Sensitivity pattern. Materials and Methods-The study was conducted in the Department of Microbiology, T.S. Misra Medical College & Hospital, Lucknow, for a duration of 1 year . 1200 pus were studied. Staphylococcus aureus with other bacteria were isolated and antibiotic profile was determined using standard protocol. Result- Total 214(47.66%) Staphylococcus aureus were isolated from 449 culture positive samples. Staphylococcus aureus showed 100% sensitivity to Linezolid and Vancomycin; 91.12% sensitivity to tetracycline, 92.52% sensitivity to Gentamicin, 85.98% sensitivity to Teicoplanin, 83.64% sensitivity to Clindamycin, 71.96% sensitivity to cefoxitin,75.23% sensitivity to Erythromycin, 68.69% sensitivity to Ciprofloxacin, 52.80% sensitivity to Chloramphenicol, 56.54% sensitivity to Cotrimoxazole, 21.49% sensitivity to Penicillin. Conclusion- Staphylococcus aureus is one of the common organism found in pus samples with increasing drug resistance. So appropriate use of antibiotics is very crucial in preventing emergence of multidrug resistance.