Background: The paleolithic diet which is a low carbohydrate diet has gained immense attention from medical professionals and the general population concerning its ability to control diabetes and weight loss with other added benefits. However, it is vital to assess its efficacy on levels of lipid and cardiovascular risk has not been answered. Aim: The present study was aimed to assess the impact of a short-term low-carbohydrate paleolithic diet on levels of lipids such as HDL (high-density lipoprotein), LDL (low-density lipoprotein), and TAG (triglycerides) along with cardiovascular risk markers such as ApoB (apolipoprotein) and ApoA1 (apolipoprotein A1). Methods: The present study assessed 212 subjects attending institute OPD for differing health concerns and were assessed after intervening with their dietary pattern. The diet prescribed to study subjects was the Paleolithic diet having a fat, carbohydrate, and protein ratio of 65:20:15, and were followed regularly for compliance. Results: Following three months of compliance, the study results showed that the paleolithic diet has a significant impact on decreasing the levels of triglycerides with p=0.001 and significantly increasing LDL, HDL, and ApoA1 levels with p=0.0008, 0.004, and 0.01 respectively. However, no significant change was seen in ApoB levels, LDL/ApoB ratio, and ApoB/ApoA1 ratio. Conclusion: The present study concludes that a short-term, low-carbohydrate paleolithic diet is not pro-atherogenic, however, it poses favorable alteration in cardiac markers and lipid profile and hence decreases the associated risk of CVD (cardiovascular disease).