Background: Strokes are the third leading cause of disability and the second leading cause of mortality worldwide.The present study was conducted to assess the association of ischaemic stroke and serum 25-hydroxyvitamin-D levels. Materials & Methods: 140 subjects of both genderswere divided into 2 groups based on stroke. Group I did while Group II did not have a stroke. A number of parameters were recorded, including body mass index (BMI), marital status, education, dietary intake, total cholesterol (TC), glycated hemoglobin (GHb), high-density lipoprotein (HDL), C-reactive protein (CRP), and 25(OH)D levels (deficiency: <30 nmol/L, insufficiency: 30–50 nmol/L, normal: 50–125 nmol/L, and adequacy: >125 nmol/L). Additionally noted was the history of diabetes mellitus, hypertension, high cholesterol, emphysema, chronic bronchitis, and drinking, whether present or not. Results: Group I had 45 males and 25 females and group II had 35 males and 35 females. There were 32 married, 20 unmarried and 18 divorced in group I and 46 married, 22 unmarried and 2 divorced in group II. Education was more than high school in 32 and 30 and less than high school in 38 and 40 patients in group I and II respectively. Diabetes mellitus was positive in 52 and 10 patients in group I and II respectively. Emphysema was positive in 45 and 26 patients in group I and II respectively. Hypertension was positive in 57 and 21 patients in group I and II respectively. Chronic bronchitis was positive in 23 and 16 patients in group I and II respectively. The difference was significant (P< 0.05). The mean 25(OH)D nmol/L level was deficient in 14 in group I and 5 in group II, insufficiency in 27 in group I and 16 in group II, normal in 21 in group I and 34 in group II, and adequate in 8 in group I and 15 in group II patients respectively. Conclusion: Serum 25(OH)D deficiency may be associated with an increased risk of stroke. Serum 25(OH)D at admission is useful prognostic marker of CVD and all-cause mortality in patients with ischemic stroke.