Background: Owing to the counter-regulation of Th1 and Th2 cells, Th1-type autoimmune diseases such as autoimmunity of thyroid and Th2-mediated allergic diseases such as atopic dermatitis must be seen in mutually exclusive subjects. However, thyroid autoimmunity is associated with chronic urticaria, and atopy is considered a cause of both urticaria and AD (atopic dermatitis). Aim: The present study was aimed to assess the frequency of thyroid autoimmunity in child subjects with atopic dermatitis and evaluate the relationship in two conditionsutilizing the SCORing Atopic Dermatitis (SCORAD) score, and biochemical parameters of serum immunoglobulin E (IgE), absolute eosinophil count, and vitamin D levels. Methods: The present study assessed child subjects with atopic dermatitis aged 0-18 years. It excluded subjects with immunodeficiency disorder, sick euthyroid syndrome, and drugs affecting thyroid dysfunction. SCORAD was used to assess the clinical disease severity. Other parameters assessed were vitamin D levels, serum IgE, absolute eosinophil count, ANA (antinuclear antibody), anti-thyroid peroxidase antibodies, and thyroid profile. Results: The study showed that thyroid autoimmunity was seen in 18.9% (n=20) subjects among 106 subjects. A significant correlation was seen in serum IgE levels and SCORAD with p=0.002 and SCORAD and absolute eosinophil counts with p<0.001. A significant negative correlation was seen in vitamin D levels and SCORAD with p=0.006. Conclusions: The present study concludes that thyroid autoimmunity can be linked with atopic dermatitis making a high index of suspicion vital. Vitamin D should also be supplemented in children with atopic dermatitis as it is commonly low, particularly in severe cases. However, future multi-center case-control studies are warranted to assess the prevalence of thyroid autoimmunity in children with atopic dermatitis.