Background: Milk production may refer to the volume of milk removed from the breast at a breastfeeding session or expression; it may also refer to the average volume of milk removed per unit of time such as milliliters in a 24-hour period.The present study was conducted to evaluate the impact of music therapy on amount of breast milk secretion among mothers of premature newborns. Materials & Methods: 60 mothers of premature babies were selected. Over the course of four days, each subject underwent four sessions of music therapy (MT) (Group I) and four sessions of no music therapy (NMT) (Group II). Mil secretion, psychological stress and the salivary cortisol level was assessed. Results: The mean milk secreted on day 1 in group I was 6.3 ml and in group II was7.3 ml. On day 2 was 6.5 ml and 6.8 ml, on day 3 was 6.9 ml and 7.2 ml and on day 4 was 7.2 ml and 7.9 ml respectively. The difference was significant (P< 0.05). The mean PSS on day 1 was 41.5 and 32.1 and on day 4 was 32.6 and 24.8 in group I and II respectively. Salivary cortisol level on day 1 was 3.42 and 3.01 and on day 4 was 2.91 and 2.14 in group I and II respectively. The difference was significant (P< 0.05). Conclusion: Mothers of hospitalized premature newborns benefit from music therapy because it lowers stress levels, which increases the amount of breast milk produced.