Background:Hyperglycemia or abnormally high blood sugar is a hallmark of a set of metabolic diseases commonly referred to as diabetes mellitus (DM). The present study was conducted to assess MDA, creatinine and Cystatin C level in patients with diabetes mellitus.Materials & Methods: 53 diabetic patients of both genders were put in group I and group II had 53 healthy subjects. The level of malondialdehyde (MDA), Cystatin C and creatinine levels were measured in both groups. Results: Group I had 30 males and 23 females and group II had 26 males and 27 females. Cystatin C level was 0.81 mg/L in group I and 0.89 mg/L in group II. MDA level was 4.08 µM in group I and was 1.34 µM in group II. Creatinine level was 0.97 mg/L in group I and 0.82 mg/L in group II. The difference was significant (P< 0.05).Conclusion: Compared to healthy persons, patients with diabetes mellitus had higher levels of MDA.