Background: Pseudoexfoliation syndrome (PXF) is a age related, chronic, ubiquitous abnormal accumulation of flaky fibrillary material at the anterior lens capsule, iris and pupillary margin ,corneal endothelium, angles of the eye and various other structures of the anterior segment of the eye. Substantial research has determined the effect of PXF syndrome without glaucoma on central corneal thickness (CCT).Aim: The aim is to determine the existence of variation in diurnal Central Corneal Thickness (CCT) and Intraocular Pressure ( IOP) between eyes with Pseudoexfoliation syndrome ( PXF ) without glaucoma, Primary Open Angle Glaucoma(POAG) and eyes of normal patients with no existing ocular pathology and to determine its effects on the progression of the disease.Methods: Case control cross sectional analytical study. 63 patients with PXF without glaucoma, POAG and aged matched normal were taken. CCT and IOP measurements were taken in all patients throughout the day and variances were noted.Results: There was a larger variance in the CCT of the PXF group as compared to the normal population and this result was statistically significant. The variances in the POAG group and the PXF group were also similar however we could not correlate this significantly. We also analyzed the variance in CCT with IOP measurements in each group and across groups and found that PXF group had significant fluctuation in IOP when correlated with fluctuations in CCT.Conclusion: Patients with Pseudoexfoliation without glaucoma must undergo periodic monitoring of CCT and IOP for early detection of glaucomatous damage for prompt treatment to prevent further glaucomatous damage and progression of the disease.