Introduction: The anatomical study of roots and canals has both clinical and anthropological significance. A thorough understanding of the root canal shape is essential for successful root canal therapy. Objective: The main objective of this study is to find the advanced analysis of third mandibular molar root anatomy using cone beam computed tomography (CBCT). Methodology: This retrospective study was conducted and data were collected from 150 patients. Demographic data, age, gender, socioeconomic status and medical history were collected through a systematically designed questionnaire. The questionnaire consisted of questions related to demographic data of patients. The CBCT scans of 150 patients were then assessed with CS3D imaging software (NNT) with a resolution of 1280 x 1024 pixels. Results: Data were collected from 150 patients from both genders according to criteria. Mean age of the patients was 45.7 ± 12.3 years. The results demonstrate a significant correlation between age and various anatomical parameters of mandibular third molars. Younger individuals (18-30 years) exhibited longer root lengths (16.5 ± 1.2 mm) and larger root canal diameters (1.8 ± 0.3 mm) compared to older age groups (>61 years), where root lengths decreased to 14.0 ± 1.5 mm and root canal diameters decreased to 1.4 ± 0.2 mm. Teeth located mesially demonstrated the longest mean root length (15.8 ± 1.2 mm) compared to distal, buccal, and lingual positions. Additionally, mesially positioned teeth exhibited a higher mean number of roots (1.9 ± 0.4) and root canals (2.6 ± 0.5) compared to other positions. Conclusion: It is concluded that age is a significant factor associated with anatomical features of mandibular 3rd molar because younger patients exhibiting longer root length and larger root canal diameter as compared to older patients. Demographic factors such as ethnicity and age influence root morphology, highlighting the need for tailored treatment approaches in dental practice.