Introduction: Substance abuse among women is an emerging concern, particularly in urban settings where changing social dynamics and living arrangements influence lifestyle choices. This study assesses substance abuse among women residing in Paying Guest (PG) accommodations in Bengaluru, a rapidly urbanizing city in India. The research aims to explore the prevalence, patterns, and factors contributing to substance use in this unique residential setting. Methodology: A cross-sectional survey was conducted among 290 women, living in various PG accommodations across Bengaluru. Data were collected using a structured questionnaire focusing on demographic details and substance use behaviour. Results: Among 290 study subjects, 82 (28.27%) had a history of taking alcohol, 31 (10.68%) had history of use of tobacco products, 6 (2.06%) had a history of cannabis use and 3 (1.03%) had history of use of others psychoactive substances such as inhalants, hallucinogens, and opioids. Conclusion: The prevalence of substance use, particularly alcohol and tobacco, underscores the need for targeted interventions to address the underlying factors. Promoting mental health support, increasing awareness about the risks of substance abuse, and implementing stricter regulatory measures are crucial steps in mitigating this issue.