Background: Among the most widespread ailments in humans are UTIs. Fosfomycin is one such drug that is once again being used to treat UTIs in the United Kingdom (UK).The present study was conducted to assess sensitivity pattern of fosfomycin against UTI pathogens. Materials & Methods: 74 patients with urinary tract infection (UTI) of both genders were selected. All patients had midstream clean catch urine samples taken in a sterile urine container. Every urine sample was semi-quantitatively plated on CLED agar and left to incubate overnight at 37°C. Tests for antibiotic susceptibility were performed on the notable increases in harmful microorganisms. The Kirby-Bauer disc diffusion test was performed using a 200 µg disc of fosfomycin. Results: Out of 74 patients, 34 were males and 40 were females. Organism isolated were E. coli in 58, Enterococcus spp. in 6, Klebsiella spp. in 3, Pseudomonas spp. in 3, Enterobacter spp. in 2, and Acinetobacter spp. in 1 and Proteus spp.in 1 case. E. coli showed maximum susceptibility against FOS (48) followed by NIT (37). Enterococcus spp. showed maximum susceptibility against FOS (9) followed by CZ (6). Klebsiella spp. showed maximum susceptibility against FOS (3), and FQ (3). Proteus spp. showed maximum susceptibility against FOS (2). Pseudomonas spp. showed maximum susceptibility against FOS (3), and Acinetobacter spp. showed maximum susceptibility againstshowed maximum susceptibility against NIT (2), and CZ (2). Conclusion: As part of a patient's empirical treatment arsenal for UTIs, fosfomycin trometamol should be used in conjunction with nitrofurantoin due to its low MIC distribution and high susceptibility.