Aims and Background: Adverse effects of varieties of culturally integrated form of tobacco on periodontium have been studied in the recent era. This cross-sectional study was aimed at possible relationships between the effects of different tobacco habits on periodontal status in the rural population of Western Maharashtra. Materials and Methods: This study comprised of total 5006 subjects (Male-3590; Females-1416). Clinical examination comprised of an intra-oral examination with specific emphasise to attachment loss, pocket depth, mobility, furcation involvement and subject to a detailed questionnaire in order to obtain information regarding the tobacco habits. Results: Results shows that tobacco users have more severe periodontitis as compared to non-users. Mishri (type of smokeless tobacco) is commonly used as an oral hygiene practice. Logistic regression analysis, after adjusting for Oral hygiene index-simplified, further proves a strong correlation between tobacco usage and periodontitis. Interesting finding is that in this rural population smokeless tobacco habit is more as compared to smoking tobacco habit. Conclusion: The findings of this study highlight the importance of stoppage of tobacco habit to improve periodontal health status among the rural population in India. Clinical significance: Proper implementation of law and patient’s education regarding ill effects of tobacco and proper tooth brushing techniques are required.