Background: The introduction of mesh marked a significant advancement in hernia surgery. It is theorized that mechanical mesh fixation techniques may result in greater tissue damage compared to nonmechanical approaches. This study was designed to evaluate the clinical outcomes of mesh fixation using fibrin glue, a nonmechanical method, compared with tackers in totally extraperitoneal (TEP) repair of unilateral inguinal hernia. Materials and Methods: A single-blinded, randomized controlled trial was conducted on individuals diagnosed with unilateral, uncomplicated inguinal hernia, with a hernial sac measuring no more than 5 cm, were eligible for inclusion. A total of 78 participants were randomized into two groups prior to mesh fixation. In group A, mesh fixation was performed with tackers, whereas in group B, fibrin glue was employed for the procedure. Patients were followed up prospectively for a period of three months. Results: No statistically significant differences were observed between the groups regarding operative duration. However, the fibrin glue group demonstrated shorter average hospital stays. Postoperative complications, including urinary retention, seroma, and hematoma formation, showed no notable differences between the groups. Patients in the fibrin glue group resumed their regular activities earlier compared to those in the tacker group. Conclusion: Considering the superior outcomes and cost-effectiveness associated with the fibrin glue fixation technique, it is recommended as a preferable alternative to tackers for mesh fixation in TEP repair of hernias.