Background: Vaccination has played a pivotal role in preventing vaccine preventable diseases and is an integral part of the overall health system. Nowadays the phenomenon of “vaccine hesitancy” is increasing among a relevant part of the world population.1,2 Though most often a positive attitude toward vaccination is seen, many medical students show lacunae in their own immunization histories and inadequate knowledge about vaccination.3 The World Health Organization’s Global Strategy on Human Resources for Health: Workforce 2030sets policy recommendations and targets for in-service and pre-service training programs to improve workforce competency which includes outcomes on improving competencies and behavior change for immunization service delivery and student readiness for immunization practice.4 Evidence-based observations also demonstrate that strong provider recommendations increase vaccine confidence in patients. As future clinicians, these students are primary influencers and sources of information on vaccination for the public.5It is therefore important to assess the knowledge and attitude of fresher medical students towards vaccination and train them accordingly. It has been demonstrated that multidisciplinary formative interventions comprise the most powerful strategy to improve knowledge about vaccines, educational intervention being foremost among these.6,7 Aim: To bring about an increase in the knowledge and awareness level about vaccination in fresher medical students, thereby contributing to vaccine acceptance and decreasing vaccine hesitancy. Objective: To assess the outcome of educative session regarding vaccination on perceptions and awareness of fresher medical students. Methodology: Aqualitative, quasi experimental study was conducted for fresher medical students, which included both pre session & post- session, using validated structured questionnaires. Statistical analysis was carried out using Chi-squared and MC nemar tests & results were considered statistically significant when the p value obtained was lower than 0.05. Results:A total of 140 students participated in the study. Overall, the level of knowledge on vaccinations changed significantly, while perceptions also were more positive after educational intervention. Awareness: 128 (91.4%) of them were aware of their own immunization history and had been vaccinated, while 11 (8%) were unaware. 51 (36%) were sure of having a record of their immunization, with 44 (32%) being not very sure. Knowledge & perceptions: Improvement in perception positivity was seen in recognizing the contribution of vaccines in the context of infectious diseases [133 (95%) to 136 (97%)] and safety of vaccines [108 (77%) to 129 (92.3%)]. Conclusion: Educative interventions for medical students, when started early, can have a highly positive impact on knowledge and attitudes of these students regarding vaccinations.