Introduction: Acinetobacter species is a leading cause of nosocomial pathogen in hospital set up worldwide. Acinetobacter baumannii is responsible for an increasing number of cases of blood stream infections, urinary tract infections, wound infections, health care & ventilator associated pneumonia. Due to surfacing of multidrug resistant strains in recent era, there is rising of morbidity and mortality rates particularly in developing countries. Material and methods: A retrospective study conducted for 6 months period. Total 17680 samples received from various departments were included. Among total samples 2598 samples showed culture positive. Acinetobacter baumannii were identified by using standard operating procedures. Kirby Bauer Disc Diffusion Method was performed as per Clinical Laboratory Standards Institute (CLSI) guidelines. Results: Out of 2598 (14.69%) culture positive samples, Acinetobacter baumannii were 129 (4.97%). No gender preponderance was observed. Acinetobacter baumannii was isolated maximum from blood (9.20%), followed by urine (5.90%), pus (4.90%), fluids (4.20%), sputum (2.10%) and cervical/vaginal swabs (0.90%). Highest sensitivity has been observed to Imipenem, Amikacin and least sensitivity for Cotrimoxazole, Fluroquinolones. Conclusion: High frequency of drug resistance to commonly used antibiotics was observed. Surveillance should be done periodically in hospitals and their antibiotic policy should be updated at regular intervals. Infection and Prevention Control measures such as hand hygiene & strict standard precautions shall be implemented to prevent nosocomial infections.