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Volume 13 Issue 12 (December) 2024

Original Articles

Determination of NS1, IgG and IgM profile during an outbreak of Dengue at Tertiary Care Teaching Hospital, Doda, Jammu & Kashmir
Samera Akhtar, Lakbir Singh, Kamaljeet, Shrutika Kumawat, Mohit Thalquotra

The present study is based on the assesment of fast of Immunochromatography test (ICT) over already existing ELISA test to detect and diagnose the dengue suspected cases. The antigen and antibodies identification criteria are used to detect the dengue specific NS1 antigen, IgM AND igG antibodies. A total of 635 patients’ blood samples were collected from Government Medical College & Hospital in Doda, Jammu & Kashmir. Thepatients were showing the signs of fever for four days with established trombocytopenia (≤1,00,000 platelets/µL of blood). The similar samples were already subjected to be assessed by ELISA had given 26.0% positive rate. The similar detection pattern was reported in 98 samples checked by both the tests. Furthermore, NS1 antigen detection through ICT had displayed 57.6% sensitivity and 88.1% specificity. All the samples were detected against NS1, IgM and IgG to avoide the false positive results throughout. We propose that ICT rapid method is an excellent and ease to handle point of care method to be deployed for dengue cases screening in the outbreak areas.

 
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