Background: Obesity is a global health challenge strongly associated with dyslipidaemia, characterized by significant alterations in lipid profiles, including elevated total cholesterol (TC), triglycerides (TG), LDL cholesterol (LDL-C), and reduced HDL cholesterol (HDL-C). Understanding these biochemical changes is crucial for mitigating cardiovascular and metabolic risks. Methods: This cross-sectional study included 200 participants aged 18–60 years, divided into obese (BMI ≥30 kg/m²) and non-obese (BMI <25 kg/m²) groups. Fasting blood samples were analyzed for lipid parameters using automated clinical chemistry methods. Dietary habits, physical activity, and other demographic data were collected through structured questionnaires. Statistical analyses were performed to compare lipid profiles and examine correlations with BMI and other factors. Results: Obese individuals showed significantly higher TC (210 ± 35 vs. 180 ± 30 mg/dL), TG (180 ± 40 vs. 120 ± 25 mg/dL), LDL-C (130 ± 30 vs. 100 ± 20 mg/dL), and lower HDL-C levels (42 ± 7 vs. 55 ± 10 mg/dL) compared to non-obese individuals (p < 0.001). BMI positively correlated with TC, TG, LDL-C, and VLDL-C (p < 0.001), while HDL-C was negatively correlated. High-fat diets and physical inactivity were strongly associated with dyslipidaemia. Conclusion: These findings underscore the role of obesity in lipid metabolism alterations and associated cardiovascular risks. Lifestyle modifications, including dietary interventions and regular physical activity, are essential for mitigating dyslipidaemia. Future research should explore personalized therapeutic strategies, incorporating genetic and environmental factors.