Background:Urinary tract infections are among the most commonly encountered infections in the children.Both Gram-negative, Gram-positive bacteria andCandida species, can cause UTI. Emergence of resistance touropathogens to commonly used antibiotics for the treatment of UTI is a major challenge. Objectives: To study the incidence, risk factors, causative organisms associated with UTI and their antimicrobial susceptibility pattern in children aged 1 to 15 years. Methods: A one year hospital based cross sectional study was carried out in the department of Pediatrics BNMCC Hospital, in collaboration with the department of Microbiology, GMC, Amritsar. Urine sample was collected in a wide mouth, screw capped sterile container under aseptic precautions. The sample was processed in the microbiology laboratory as per the standard microbiological procedure. Results: In our study 2736 urine culture were done, out of which 196 (7.16%)samples were found to have significant bacteriuria.Out of the 196 culture-positive cases, 77.55% had gram-negative isolates, 16.84% had gram-positive isolates, and 4.59% had yeast (Candida) isolates. The most common organism causing UTI was Escherichia coli, found in 73.02%cases. Conclusion: The findings from this study necessitates the importance of integrating stewardship principles into routine clinical practice and ensuring the sustainability of effective antibiotic therapies for future generations.