Background: Oral cancer is the sixth most frequent type of cancer worldwide. In the Indian subcontinent, it is among the top three types of cancer.1 About 90% of cancer cases in the oral cavity are caused by squamous cell carcinoma (SCC). The present study was conducted to assess salivary biomarkers in patients with squamous cell carcinoma. Materials & Methods: 54 patients with SCC patients of both genders were put in group I and healthy control in group II. 5 ml of unstimulated saliva was collected and the saliva sample was centrifuged at 3000 rpm for 15 min to remove squamous cells and debris. Assessment of IL1b, IL8, SAT1, OAZ1 was done with PCR and LDH with standard kit method. Results: Out of 82 SCC patients, 50 were males and 32 were females. OAZ1 was 24.7 in group I and 14.2 in group II, LDH level was 412.5 in group I and 121.8 in group II, IL 1 b level was 104.2 in group I and 27.5 in group II, IL 8 was 224. 8 in group I and 29.6 in group II, SAT 1 was 26.6 in group I and 23.4 in group II. The difference was significant (P< 0.05). Conclusion: Higher concentrations of all salivary biomarkers in SCC patients' saliva than in those of healthy individuals. Saliva can therefore be used as a diagnostic tool.