Background: Hepatitis B (HBV) and Hepatitis C virus (HCV) infections are the commonest cause of viral Hepatitis among Hemodialysis (HD) patients. Present study was carried out to find out of the prevalence of viral markers like HBsAg and Anti HCV in the newly diagnosed Chronic Renal Failure patients, taken for maintenance Hemodialysis. Material and Methods: Present study was hospital based, prospective cross sectional and multicentered study, conducted in patients of All age groups, both Genders, cases of Adult CKD taken for maintenance Haemodialysis, who had not received HBV vaccination at any point earlier, free from overt infection, immunosuppression & free from any chronic disease including autoimmune disorders. Results: Among 1705 patients, there were 1189 males (69.7%) and 516 were females.(30.3%) and their mean age was 42.6 years.At the initiation of hemodialysis, among the study population two of them were reactive to HbsAg, 15 to anti HCV and five were found to be having co-infection. The prevalence of HbsAg and Anti HCV and both among the study was 0.11%, 0.87% and 0.29% respectively.The distribution of HbsAg and Anti HCV among the healthy voluntary blood donors was 1.33% and 0.9% respectively. None of them carried both HbsAg and Anti HCV during the study period.Distribution of 22 males and 12 females on MHD carried one or other viral markers or both viral markers. HCV genotyping assay determined the Genotypes 1, 2,3,4,5 & 6 for the Anti HCV positive case, revealed that it belonged to genotype 3b. Conclusion: Seroconversion at the end of 3 months of MHD among the cases who were negative for HbsAg and Anti HCV was 0.25 and 0.5% respectively. Genotype 3b was identified in 16 (57.1%) of 28 cases (42.9%) and 1b in rest of the cases.