TB is the leading cause of death from a single infectious agent in the world. The advent of MDR-TB has further complicated the situation. The use of culture-based drug resistance testing is hindered by unacceptable long turn around for results and high contamination rates. The technological difficulties of conducting a TB culture test causes a problem for case diagnosis and initiation of treatment.This is a prospective cross sectional, descriptive study conducted among the sputum positive presumptive DR-TB patients.The study was conducted over a period of one year. The sensitivity and specificity of GeneXpert MTB/RIF for the detection of Rifampicin mono resistance in our study was 60% and 94% and for LPA it was found to be 100% and 100% respectively. Further analysis of samples by MGIT-DST showed discrepancy between LPA and GeneXpert results, confirms 100% agreement between MGIT 960 and LPA. LPA has a better efficiency characteristic than GeneXpert and an alternative to culture for the diagnosis of RIF mono-resistance.