Background: Acne vulgaris is a common skin condition that is characterized by cysts, pimples, whiteheads, and blackheads. Though the results have been mixed, recent dermatological research has investigated the role of magnesium (Mg) and zinc (Zn) in acne. This study's objective is to investigate how serum zinc plus magnesium levels in male Indian patients relate to the severity of their acne. Additionally, it compares these levels to those of healthy people in order to determine how they might relate to acne development. Method: The comparison analysis was carried out in the department of Dermatology and Venereology at NC Medical College and Hospital, Israna, Panipat, Haryana There were 150 participants in all, 75 of whom had acne vulgaris and 75 of whom were healthy controls. All participants were between the ages of 20 and 35. In order to further categorize the acne patients, three groups were created: mild (25 patients), moderate (25 patients), and severe (25 patients). To determine their possible involvement in acne, serum levels of magnesium (Mg) and zinc (Zn) were assessed together in the patient and healthy subjects. Results: Acne subjects (all severities united) and the healthy control group did not significantly differ in their average serum zinc (Zn) plus magnesium (Mg) levels. On the other hand, patients with mild acne (P = 0.00003) and those with severe acne (P = 0.004) had significantly lower zinc levels. The groups with severe and moderate acne did not differ statistically significantly (P = 0.78). Additionally, the magnesium levels of the severe acne group were significantly lower than those of the mild acne group. Conclusion: This study discovered a strong correlation between the severity of acne and serum levels of magnesium and zinc. Zinc supplementation may increase the efficacy of acne treatment in patients with low zinc levels.