Background: Obstructive sleep apnea (OSA) is a common sleep disorder characterized by repetitive upper airway obstruction during sleep, resulting in fragmented sleep patterns and reduced oxygen saturation. OSA is associated with significant comorbidities such as hypertension, diabetes, and cardiovascular conditions. This study aimed to evaluate the diagnostic profile of OSA patients through clinical, physical, and polysomnographic assessments. Methods: This observational study was conducted at the Department of Otorhinolaryngology, Civil Hospital, Parbhani, from June 2017 to October 2018. A total of 70 patients suspected of OSA were evaluated based on clinical history, physical examination, and full-night polysomnography. Patients were assessed for symptoms such as snoring, excessive daytime sleepiness (EDS), and disturbed sleep. Video endoscopy with Muller’s maneuver to identify the site of obstruction were. Comorbidities such as hypertension, diabetes, and hypothyroidism were recorded. Results: The majority of the study population were aged between 41-50 years (38.57%) with a slight male predominance (55.71%). Snoring (92.86%), EDS (82.86%), and disturbed sleep (85.71%) were the most commonly reported symptoms. Modified Mallampati Grade III (42.86%) was the most common, while tonsillar hypertrophy was prominent in 34.29% (Grade II). The retropalatal region was the most frequently identified site of obstruction (34.29%), with 42.86% of patients demonstrating multi-level airway obstruction. Conclusion: The study highlights the significant burden of OSA among middle-aged individuals with a high prevalence of snoring, EDS, and disturbed sleep. The findings reinforce the role of obesity, increased neck circumference, and multi-site airway obstruction in the pathogenesis of OSA. Comprehensive evaluation using polysomnography and video endoscopy is crucial for accurate diagnosis and treatment planning. Early identification and intervention strategies can help reduce the associated morbidity and improve patient outcomes.