Introduction: Parkinson's Disease (PD) is one of the most common neurodegenerative disorders, mostly affecting elderly people. It is caused by the degeneration of dopaminergic neurons in the striata nigra. Allium sativum and Phyllanthus emblica are known to possess anti-inflammatory, antioxidant, and neuroprotective properties. Thus, the study was designed to assess the neuroprotective effect of A. sativum and P. emblica in the rotenone-induced Parkinson's model of Drosophila melanogaster. Materials and Method:Drosophila flies were cultured in cornmeal agar medium. Seven-day-old flies were divided into five groups with approximately 30 flies in each group: normal control, disease control, Levodopa (1 mM), A. sativum (0.125% and 0.25%), and P. emblica (0.25% and 0.5%). Rotenone (125 µM) was used to induce the PD. All drugs were administered through the cornmeal agar medium for seven days, and on the eighth day, a climbing assay was performed. Effect on biochemical variables like Malondialdehyde and Dopamine levels in the Drosophila brain were also assessed. Result: There was a significant improvement in the flying and climbing ability of Drosophila flies in all the A. sativum and P. emblica treated groups compared to the disease control group. They also significantly decreased MDA levels and improved dopamine levels in the brain. Among all these groups, the high dose of P. emblica (0.5%) was found to be maximally effective. Conclusion: A. sativum and P. emblica were found to be neuroprotective as they improved the locomotor activity of Drosophila flies, probably by reducing oxidative stress, leading to the prevention of degeneration of dopaminergic neurons.