Background and Objectives: Antiphospholipid syndrome (APS) has been reported to be one of the most important causes of Recurrent Pregnancy Loss (RPL). In this study, we compared the prevalence of APS in women with and without history of RPL. Methods: A total of 30 women in reproductive age group (15-45) years with a history of three or more consecutive pregnancy loss upto 20 weeks of gestation were enrolled as cases and a total of 15 age- matched women with at least one successful pregnancy and no history of pregnancy loss were enrolled as controls. Women with history of still birth or intermittent pregnancy loss were excluded. Blood specimen from all the women were obtained and assessed for activated partial thromboplastin time (APTT), Dilute Russell’s viper-venom test (DRVVT), Anticardiolipin, β-2 glycoprotein 1 and antiphospholipid antibody tests. Data was compared between cases and controls using Fisher exact test. Results: Mean age of cases and controls was 29.37±5.79 and 28.60±4.31 years respectively (p>0.05). Cases had an average of 3.33 pregnancy losses. Prevalence of APS was 36.7% in cases as compared to 0% in controls (p=0.008). None of the cases or controls had elevated APTT. Elevated DRVTT was the least common abnormality in cases (3.33%) whereas β-2 glycoprotein antibody was the most common abnormality (36.7%). At factorial level, except for β-2 glycoprotein antibody (p=0.008), none of the other APS factors showed a significant difference between cases and controls (p>0.05). Conclusion: APS induced thrombosis could be one of the most important causes of RPL.