Background: Type-II Diabetes Mellitus (T2DM) is a metabolic disorder characterized by insulin resistance, β-cell dysfunction, and chronic hyperglycemia. Emerging evidence suggests that oxidative stress plays a crucial role in T2DM progression by contributing to cellular damage, lipid peroxidation, and chronic inflammation. However, the correlation between oxidative stress biomarkers and diabetes severity remains underexplored. Objective: This study aims to: • Investigate the role of oxidative stress in T2DM pathogenesis. • Analyze oxidative stress biomarkers (malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT)) in diabetic and non-diabetic individuals. • Evaluate the impact of antioxidant therapy and lifestyle interventions on oxidative stress in T2DM. Methods: A case-control study was conducted at Index Medical College, Hospital & Research Center, Indore, under Malwanchal University, from 2022 to 2024. 100 T2DM patients and 100 age- and sex-matched healthy controls were recruited. Blood samples were analyzed for oxidative stress markers, glycemic parameters, lipid profile, and inflammatory markers. Antioxidant therapy (Vitamin C, E, alpha-lipoic acid) and dietary interventions were assessed over 12 weeks. Data were analyzed using SPSS v.26, with statistical significance set at p < 0.05. Results T2DM patients exhibited significantly higher MDA and NO levels and lower SOD and CAT activity (p < 0.001). MDA correlated positively with HbA1c (r = 0.72, p < 0.001), indicating oxidative stress-induced glycemic dysregulation. Antioxidant therapy significantly reduced MDA levels and improved SOD activity (p < 0.01). Conclusion: Oxidative stress plays a critical role in T2DM progression. Monitoring oxidative stress biomarkers and implementing antioxidant-based therapies may improve diabetes management and reduce complications.